• Advancing T cell–based cancer therapy with single-cell technologies, Nature Medicine
• High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails, Nature Biotechnology
• RASA2 ablation in T cells boosts antigen sensitivity and long-term function, Nature
• Systematic discovery and perturbation of regulatory genes in human T cells reveals the architecture of immune networks, Nature Genetics
• Global post-translational modification profiling of HIV-1-infected cells reveals mechanisms of host cellular pathway remodeling, Cell Reports
• Hypoxia Is a Dominant Remodeler of the Effector T Cell Surface Proteome Relative to Activation and Regulatory T Cell Suppression, Molecular & Cellular Proteomics
• Obesity alters pathology and treatment response in inflammatory disease, Nature
  • Research Highlight in Nature Reviews Immunology
• A functional map of HIV-host interactions in primary human T cells, Nature Communications
• CRISPR activation and interference screens decode stimulation responses in primary human T cells, Science
• CRISPR–Cas9-mediated nuclear transport and genomic integration of nanostructured genes in human primary cells, Nucleic Acids Research

• Efficient Generation of Isogenic Primary Human Myeloid Cells using CRISPR-Cas9 Ribonucleoproteins, Cell Reports
• XYZeq: Spatially resolved single-cell RNA sequencing reveals expression heterogeneity in the tumor microenvironment, Science Advances
• Pooled screening of CAR T cells identifies non-native signaling domains for next-generation immunotherapies, bioRxiv
• Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing, bioRxiv

• Evaluation of SARS-CoV-2 serology assays reveals a range of test performance, Nature Biotechnology
• Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies, Cell
• Functional CRISPR dissection of gene networks controlling human regulatory T cell identity, Nature Immunology
• CRISPR screen in regulatory T cells reveals modulators of Foxp3, Nature
• Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency, Nature Biotechnology
• Rewired signaling network in T cells expressing the chimeric antigen receptor (CAR), EMBO J
• Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair, Nature Communications
• T cell subset-selective IL2RA enhancers shape autoimmune diabetes risk, bioRxiv

• Landscape of stimulation-responsive chromatin across diverse human immune cells, Nature Genetics
• Orthotopic replacement of T-cell receptor alpha and beta chains with preservation of near-physiological T-cell function, Nature Biomedical Engineering
• CRISPR-Based Tools in Immunity, Annual Review of Immunology
• Helios enhances the preferential differentiation of human fetal CD4+ naive T cells into regulatory T cells, Science Immunology
• A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells, Immunity
• Large dataset enables prediction of repair after CRISPR-Cas9 editing in primary T cells, Nature Biotechnology
• A large CRISPR-induced bystander mutation causes immune dysregulation, Communications Biology
• CRISPR-Cas9 genome engineering of primary CD4 + T cells for the interrogation of HIV-host factor interactions, Nature Protocols

• Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function, Cell, 2018 | Learn More
• Reprogramming human T cell function and specificity with non-viral genome targeting, Nature, 2018 | Learn More
• CRISPR-Cas9 genome engineering of primary CD4 + T cells for the interrogation of HIV-host factor interactions, Nature Protocols, 2018 | Learn More
• Multiplexed droplet single-cell RNA-sequencing using natural genetic variation, Nature Biotechnology, 2018 | Learn More
• Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins, Journal of Immunological Methods, 2018 | Learn More

• Discovery of stimulation-responsive immune enhancers with CRISPR activation, Nature, 2017 | Learn More
• A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors, Nature Genetics, 2017 | Learn More
• CRISPR/Cas9-mediated PD-1 disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells, Scientific Reports, 2017 | Learn More
• Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements, Nature Genetics, 2017 | Learn More
• “T-bet”-ing on autoimmunity variants, PLOS Genetics, 2017 | Learn More

A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells, Cell Reports, 2016 | Learn More

• Genetic and epigenetic fine mapping of causal autoimmune disease variants, Nature, 2015 | Learn More
• The chromatin-modifying enzyme Ezh2 is critical for the maintenance of regulatory T cell identity after activation, Immunity, 2015 | Learn More
• Generation of knock-in primary human T cells using Cas9 ribonucleoproteins, PNAS, 2015 | Learn More
• Genetic basis of autoimmunity, The Journal of Clinical Investigation, 2015 | Learn More

• Small-molecule ROR-gamma-t antagonists inhibit T helper 17 cell transcriptional network by divergent mechanisms, Immunity, 2014 | Learn More
• Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells, Cell, 2008 | Learn More
• Wnt signaling promotes reprogramming of somatic cells to pluripotency, Cell Stem Cell, 2008 | Learn More
• Foxp3 occupancy and regulation of key target genes during T-cell stimulation, Nature, 2007 | Learn More